Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27939
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dc.contributor.authorAustin, A.en
dc.contributor.authorDillon, N.en
dc.date.issued1997en
dc.identifier.citationAustralian Journal of Entomology, 1997; 36(3):241-244en
dc.identifier.issn1326-6756en
dc.identifier.issn1440-6055en
dc.identifier.urihttp://hdl.handle.net/2440/27939-
dc.description.abstractTwo species of parasitic wasp, Venturia canescens and Leptomastix dactylopii, were dried from alcohol using a range of methods proposed as chemical alternatives to critical point drying, i.e. hexamethyldisilazane (HMDS), amyl acetate, xylene, methyl cellusolve and acetone vapour. Also, fresh specimens of V. canescens were dried using acetone vapour, first as a fixative and then as a drying agent. Total genomic DNA was subsequently extracted and a 524 bp fragment of the mitochondrial 16S ribosomal RNA gene amplified by PCR. This indicated that all of these drying techniques yielded high-quality DNA which was amenable to PCR. the success of chemicals like HMDS as alternate rapid drying methods for wasps and other insects means that they are likely to replace critical point drying (CPD) of museum specimens in the near future. Importantly, the results from this study show that specimens, dried from alcohol using chemical techniques, are a good potential source of DNA for molecular systematics projects.en
dc.language.isoenen
dc.publisherAUSTRALIAN ENTOMOLOGICAL SOCen
dc.source.urihttp://www3.interscience.wiley.com/journal/119177523/abstracten
dc.titleExtraction and PCR of DNA from parasitoid wasps that have been chemically drieden
dc.typeJournal articleen
dc.identifier.rmid0030002549en
dc.identifier.doi10.1111/j.1440-6055.1997.tb01461.xen
dc.identifier.pubid66555-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Agriculture, Food and Wine publications
Environment Institute Leaders publications

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