Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/28068
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Type: Journal article
Title: Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection
Author: Brown, J.
Ogunniyi, A.
Woodrow, M.
Holden, D.
Paton, J.
Citation: Infection and Immunity, 2001; 69(11):6702-6706
Publisher: Amer Soc Microbiology
Issue Date: 2001
ISSN: 0019-9567
1098-5522
Editor: Tuomanen, E.I.
Statement of
Responsibility: 
Jeremy S. Brown, A. David Ogunniyi, Matthew C. Woodrow, David W. Holden and James C. Paton
Abstract: There has been considerable recent research into protein based Streptococcus pneumoniae vaccines as alternatives to the existing capsular antigen vaccines. PiuA and PiaA (formerly Pit1A and Pit2A) are recently identified lipoprotein components of S. pneumoniae iron uptake ABC transporters which are required for full virulence and are likely to be expressed on the surface of the bacterial cell membrane. We investigated the efficacy of recombinant PiuA and PiaA proteins at eliciting protective immunity in mice against systemic infection with S. pneumoniae. Both recombinant PiuA and PiaA generated antibody responses that cross-reacted with each other but not with pneumolysin and reacted with identical proteins from nine different S. pneumoniae serotypes. Mice immunized with recombinant PiuA and PiaA were protected against systemic challenge to a degree similar to those immunized with an existing protein vaccine candidate, PdB (a genetically modified pneumolysin toxoid). Immunization with a combination of both PiuA and PiaA resulted in additive protection and was highly protective against systemic infection with S. pneumoniae. PiuA and PiaA are therefore promising additional candidates for a novel S. pneumoniae vaccine using protein antigens.
Keywords: Animals
Mice, Inbred BALB C
Mice
Streptococcus pneumoniae
Pneumococcal Infections
Iron
Sodium Dodecyl Sulfate
Lipoproteins
Histidine
Bacterial Proteins
ATP-Binding Cassette Transporters
Recombinant Fusion Proteins
Vaccines, Synthetic
Pneumococcal Vaccines
Antibodies, Bacterial
Antigens, Bacterial
Immunization, Passive
Vaccination
Electrophoresis, Polyacrylamide Gel
Rights: © 2001, American Society for Microbiology.
DOI: 10.1128/IAI.69.11.6702-6706.2001
Published version: http://iai.asm.org/cgi/content/abstract/69/11/6702
Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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