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|Title:||A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography|
|Citation:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2001; 761(2):167-175|
|Publisher:||Elsevier Science BV|
|Abstract:||A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.|
Chromatography, High Pressure Liquid
Sensitivity and Specificity
Reproducibility of Results
|Appears in Collections:||Aurora harvest 6|
Molecular and Biomedical Science publications
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