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Type: Journal article
Title: A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography
Author: Li, J.
Milne, R.
Nation, R.
Turnidge, J.
Coulthard, K.
Johnson, D.
Citation: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2001; 761(2):167-175
Publisher: Elsevier Science BV
Issue Date: 2001
ISSN: 1570-0232
Abstract: A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.
Keywords: Humans
Fluorescent Dyes
Chromatography, High Pressure Liquid
Sensitivity and Specificity
Reproducibility of Results
Mass Spectrometry
DOI: 10.1016/S0378-4347(01)00326-7
Appears in Collections:Aurora harvest 6
Molecular and Biomedical Science publications

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