Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28088
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Coates, N. | - |
dc.contributor.author | McColl, S. | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Journal of Immunology, 2001; 166(8):5176-5182 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.issn | 1550-6606 | - |
dc.identifier.uri | http://hdl.handle.net/2440/28088 | - |
dc.description | Copyright © 2001 by The American Association of Immunologists | - |
dc.description.abstract | Members of the chemokine gene superfamily are known to play a central role in leukocyte extravasation; however, their involvement in acute inflammation in response to micro-organisms has not yet been well studied. We have therefore investigated the role of murine macrophage-inflammatory protein (muMIP) 1 and muMIP-2 in the inflammatory response mounted against the bacteria Salmonella enteritidis and the Sacchromyces cerevisiae cell wall component, zymosan. Leukocyte extravasation was monitored in murine s.c. air pouches. Both agonists induced accumulation of leukocytes in a dose- and time-dependent manner, with the response peaking after 4 h and declining thereafter. The inflammatory exudate comprised mainly neutrophils; however, an increase in eosinophil accumulation was also observed in response to zymosan. The production of both muMIP-1 and muMIP-2 increased with time in response to both the agonists, although production was more sustained in response to the bacteria. Prior treatment of mice with neutralizing Abs against muMIP-1 or muMIP-2, either alone or in combination, failed to attenuate the accumulation of leukocytes in response to the agonists. In contrast, the anti-muMIP-2 Abs significantly inhibited leukocyte recruitment in response to S. enteritidis in complement-deficient mice. Taken together, these data show that while muMIP-1 and muMIP-2 are produced in response to phagocytosis of micro-organisms in s.c. tissue, under these circumstances components of the complement pathway appear to play a dominant role in the recruitment of neutrophils. | - |
dc.description.statementofresponsibility | Nicholas J. Coates and Shaun R. McColl | - |
dc.language.iso | en | - |
dc.publisher | Amer Assoc Immunologists | - |
dc.source.uri | http://www.jimmunol.org/cgi/content/abstract/166/8/5176 | - |
dc.subject | Phagocytes | - |
dc.subject | Exudates and Transudates | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Mice | - |
dc.subject | Mice, Mutant Strains | - |
dc.subject | Salmonella enteritidis | - |
dc.subject | Saccharomyces cerevisiae | - |
dc.subject | Sodium Chloride | - |
dc.subject | Phosphates | - |
dc.subject | Zymosan | - |
dc.subject | Chemokines | - |
dc.subject | Macrophage Inflammatory Proteins | - |
dc.subject | Immune Sera | - |
dc.subject | Buffers | - |
dc.subject | Diffusion Chambers, Culture | - |
dc.subject | Injections, Intraperitoneal | - |
dc.subject | Injections, Subcutaneous | - |
dc.subject | Cell Movement | - |
dc.subject | Phagocytosis | - |
dc.subject | Down-Regulation | - |
dc.subject | Complement C5 | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.subject | Chemokine CCL4 | - |
dc.subject | Chemokine CXCL2 | - |
dc.title | Production of chemokines in vivo in response to microbial stimulation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.4049/jimmunol.166.8.5176 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.