Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/281
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Type: Journal article
Title: High-affinity Rep-binding is not required for the replication of a geminivirus DNA and its satellite
Author: Lin, B.
Akbar Behjatnia, S.
Dry, I.
Randles, J.
Rezaian, M.
Citation: Virology, 2003; 305(2):353-363
Publisher: Academic Press Inc
Issue Date: 2003
ISSN: 0042-6822
2514-4138
Statement of
Responsibility: 
Baochuan Lin, S. A. Akbar Behjatnia, Ian B. Dry, John W. Randles and M. Ali Rezaian
Abstract: The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a combination of protein-binding assays and mutagenesis to show that repeat motifs in TLCV and sat-DNA are essential for Rep-binding in vitro. Surprisingly, mutants of TLCV and sat-DNA impaired in their ability to bind TLCV Rep in vitro were infectious in tomato. Thus, in contrast to other geminiviruses reported, TLCV and sat-DNA replication is independent of the high-affinity in vitro Rep binding. These results prompt a reassessment of the current model of geminivirus replication where Rep/DNA interaction is a highly specific step in the initiation of rolling circle replication.
Keywords: Tomato leaf curl virus
Rep binding
satellite DNA
DNA replication
DOI: 10.1006/viro.2002.1671
Description (link): http://www.elsevier.com/wps/find/journaldescription.cws_home/622952/description#description
Appears in Collections:Agriculture, Food and Wine publications
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