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Type: Journal article
Title: Complementation of a yeast CYC3 deficiency identifies an X-linked mammalian activator of apocytochrome c
Author: Schwarz, Q.
Cox, T.
Citation: Genomics, 2002; 79(1):51-57
Publisher: Academic Press Inc
Issue Date: 2002
ISSN: 0888-7543
Organisation: Centre for the Molecular Genetics of Development
Abstract: We have shown by ndirect immunofluorescence and enhanced green fluorescent protein fusions that a mammalian sequence exhibiting similar levels of homology to the two yeast heme lyases Cyc3p (holocytochrome c synthase; HCCS) and Cyt2p (holocytochrome c₁ synthase; HCC₁S) is also targeted to mitochondria. The human protein was able to complement the yeast Cyc3p (but not Cyt2p) deficiency, which indicates that it specifically activates apocytochrome c. Consistent with a respiratory role, expression of the mammalian gene was detected in all tissues, with the highest levels found in heart. Notably, the human gene HCCS is the only known gene located within the critical region for the deletion-defined disorder microphthalmia with linear skin defects (MLS). We believe the spectrum of clinical features seen in females with MLS and the paucity of male patients are consistent with significant involvement of HCCS. Toward clarification of a role for HCCS in disease, we have extensively characterized the X-linked mouse Hccs genomic locus, showing conservation in gene size and arrangement despite its location in a region that has undergone significant evolutionary rearrangement.
Keywords: COS Cells
Hela Cells
3T3 Cells
X Chromosome
Saccharomyces cerevisiae
Cytochrome c Group
Cytochromes c
Mitochondrial Proteins
Enzyme Activators
Sequence Alignment
Evolution, Molecular
Amino Acid Sequence
Sequence Homology
Molecular Sequence Data
Genetic Linkage
DOI: 10.1006/geno.2001.6677
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Appears in Collections:Aurora harvest 6
Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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