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dc.contributor.authorOgunniyi, A.-
dc.contributor.authorKotlarski, I.-
dc.contributor.authorMorona, R.-
dc.contributor.authorManning, P.-
dc.identifier.citationFEMS Immunology and Medical Microbiology, 2002; 34(1):23-31-
dc.descriptionThe definitive version is available at
dc.description.abstractWe have used a recombinant approach to characterise the B- and T-cell epitopes of FanC, the major subunit polypeptide of K99 (F5) fimbriae of enterotoxigenic Escherichia coli strains. This involved the fusion of FanC and its carboxy-terminal truncated derivatives to a reporter, the E. coli alkaline phosphatase (PhoA), generating stable, recombinant fusions. The B-cell epitopes of FanC were characterised by Western blotting of FanC::PhoA fusion proteins with a polyclonal mouse antiserum directed against K99 fimbrial antigen, and with a panel of monoclonal antibodies generated to the K99 antigen. An attempt to characterise the T-cell epitopes of the fimbrial subunit was made by standard in vitro T-cell proliferation assay. Our results suggest that the B-cell epitopes of FanC are likely to be continuous, with a potentially immunodominant epitope at the carboxy-terminus. However, T-cell proliferation assays with the FanC::PhoA fusion proteins did not indicate any immunodominant T-cell epitope(s). We hypothesise that fusion of FanC peptides to PhoA had resulted in altered folding of the peptides for antibody and T-cell recognition, highlighting the potential problems and drawbacks of the recombinant fusion technique in defining the epitopes of certain proteins.-
dc.description.statementofresponsibilityAbiodun D Ogunniyi, Ieva Kotlarski, Renato Morona, Paul A Manning-
dc.publisherElsevier Science BV-
dc.subjectFimbriae, Bacterial-
dc.subjectMice, Inbred BALB C-
dc.subjectEscherichia coli-
dc.subjectAlkaline Phosphatase-
dc.subjectCyclin-Dependent Kinases-
dc.subjectEscherichia coli Proteins-
dc.subjectProtein Subunits-
dc.subjectRecombinant Fusion Proteins-
dc.subjectBacterial Toxins-
dc.subjectAntigens, Surface-
dc.subjectImmunodominant Epitopes-
dc.subjectEpitope Mapping-
dc.subjectProtein Folding-
dc.titleEpitope analysis of the FanC subunit protein of the K99 (F5) fimbriae of enterotoxigenic Escherichia coli using a recombinant fusion technique-
dc.typeJournal article-
dc.identifier.orcidOgunniyi, A. [0000-0001-9308-5629]-
dc.identifier.orcidMorona, R. [0000-0001-7009-7440]-
Appears in Collections:Aurora harvest 6
Molecular and Biomedical Science publications

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