Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/28222
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Type: Journal article
Title: Pluripotent cell division cycles are driven by ectopic Cdk2, cyclin A/E and E2F activities
Author: Stead, E.
White, J.
Faast, R.
Conn, S.
Goldstone, S.
Rathjen, J.
Dhingra, U.
Rathjen, P.
Walker, D.
Dalton, S.
Citation: Oncogene, 2002; 21(54):8320-8333
Publisher: Nature Publishing Group
Issue Date: 2002
ISSN: 0950-9232
1476-5594
Organisation: Centre for the Molecular Genetics of Development
Statement of
Responsibility: 
Elaine Stead, Josephine White, Renate Faast, Simon Conn, Sherilyn Goldstone, Joy Rathjen, Urvashi Dhingra, Peter Rathjen, Duncan Walker and Stephen Dalton
Abstract: Pluripotent cells of embryonic origin proliferate at unusually rapid rates and have a characteristic cell cycle structure with truncated gap phases. To define the molecular basis for this we have characterized the cell cycle control of murine embryonic stem cells and early primitive ectoderm-like cells. These cells display precocious Cdk2, cyclin A and cyclin E kinase activities that are conspicuously cell cycle independent. Suppression of Cdk2 activity significantly decreased cycling times of pluripotent cells, indicating it to be rate-limiting for rapid cell division, although this had no impact on cell cycle structure and the establishment of extended gap phases. Cdc2-cyclin B was the only Cdk activity that was identified to be cell cycle regulated in pluripotent cells. Cell cycle regulation of cyclin B levels and Y<sup>15</sup> regulation of Cdc2 contribute to the temporal changes in Cdc2-cyclin B activity. E2F target genes are constitutively active throughout the cell cycle, reflecting the low activity of pocket proteins such as p107 and pRb and constitutive activity of pRb-kinases. These results show that rapid cell division cycles in primitive cells of embryonic origin are driven by extreme levels of Cdk activity that lack normal cell cycle periodicity.
Keywords: Cell cycle
pluripotent
stem cell
DOI: 10.1038/sj.onc.1206015
Appears in Collections:Aurora harvest 2
Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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