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|Title:||Expression of three zebrafish orthologs of human FMR1-related genes and their phylogenetic relationships|
|Citation:||Development Genes and Evolution, 2004; 214(11):567-574|
|Organisation:||Centre for the Molecular Genetics of Development|
|Tucker Ben, Richards Robert and Lardelli Michael|
|Abstract:||The human fragile X mental retardation syndrome is caused by expansions of a CGG repeat in the FMR1 gene. FXR1 and FXR2 are autosomal paralogs of FMR1. The products of the three genes, FMRP, FXR1P, and FXR2P, respectively, belong to a family of RNA-binding proteins. While the FMR1-related gene family is well described in human, mouse and Drosophila, little is known about zebrafish (Danio rerio) orthologs of these genes. Here we collate the known FMR1-related gene sequences from zebrafish, examine their regions of structural conservation, and define their orthologies with the human genes. We demonstrate that zebrafish possess only three FMR1-related genes, FMR1, FXR1 and FXR2, and these are orthologous to the human FMR1, FXR1 and FXR2 genes respectively. We examine the spatiotemporal pattern of transcription of the zebrafish genes from 0 hours post fertilisation (hpf) until 24 hpf. Expression of FMR1, FXR1 and FXR2 is widespread throughout this time. However, relative to surrounding tissues, expression of FXR2 is raised in adaxial and somitic cells by 12 hpf while FXR1 expression is high in the anterior of the embryo, and is raised in adaxial cells by 12 hpf. Distinct patterns (and levels) of expression are seen for the different genes later in development. At 24 hpf, FXR1 and FXR2 transcripts show complex distribution patterns in somites. The expression of the FMR1-related gene family in zebrafish tissues is broadly consistent with expression in mouse and human, supporting the idea that zebrafish should be an excellent model organism in which to study the functions of the vertebrate FMR1-related gene family.|
|Keywords:||Animals; Zebrafish; Humans; RNA-Binding Proteins; Nerve Tissue Proteins; In Situ Hybridization; Reverse Transcriptase Polymerase Chain Reaction; Organ Specificity; Amino Acid Sequence; Multigene Family; Molecular Sequence Data; Databases, Genetic; Fragile X Mental Retardation Protein|
|Description:||The original publication can be found at www.springerlink.com|
|Appears in Collections:||Molecular and Biomedical Science publications|
Centre for the Molecular Genetics of Development publications
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