Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/3004
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Cooperativity in long-range gene regulation by the λ CI repressor
Other Titles: Cooperativity in long-range gene regulation by the lambda CI repressor
Author: Dodd, I.B.
Shearwin, K.E.
Perkins, A.J.
Burr, T.
Hochschild, A.
Egan, J.B.
Citation: Genes & Development, 2004; 18(3):344-354
Publisher: Cold Spring Harbor Laboratory Press
Issue Date: 2004
ISSN: 0890-9369
1549-5477
Statement of
Responsibility: 
Ian B. Dodd, Keith E. Shearwin, Alison J. Perkins, Tom Burr, Ann Hochschild, and J. Barry Egan
Abstract: Effective repression of cI transcription from PRM by the bacteriophage λ CI repressor requires binding sites (OL) located 2.4 kb from the promoter. A CI tetramer bound to OL1.OL2 interacts with a tetramer bound near PRM (OR1.OR2), looping the intervening DNA. We previously proposed that in this CI octamer:DNA complex, the distant OL3 operator and the weak OR3 operator overlapping PRM are juxtaposed so that a CI dimer at OL3 can cooperate with a CI dimer binding to OR3. Here we show that OL3 is necessary for effective repression of PRM and that the repressor at OL3 appears to interact specifically with the repressor at OR3. The OL3-CI-OR3 interaction involves the same CI interface used for short-range dimer-dimer interactions and does not occur without the other four operators. The long-range interactions were incorporated into a physicochemical model, allowing estimation of the long-range interaction energies and showing the lysogenic state to be ideally poised for CI negative autoregulation. The results establish the λ system as a powerful tool for examining long-range gene regulatory interactions in vivo.
Keywords: DNA-Binding Proteins; Repressor Proteins; Viral Proteins; Lysogeny; Gene Expression Regulation, Viral; Binding Sites; Models, Biological; Operator Regions, Genetic; Viral Regulatory and Accessory Proteins
Rights: Copyright © 2004 by Cold Spring Harbor Laboratory Press.
RMID: 0020040149
DOI: 10.1101/gad.1167904
Appears in Collections:Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.