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Type: Journal article
Title: Eosinophil degranulation in the allergic lung of mice primarily occurs in the airway lumen
Author: Clark, K.
Simson, L.
Newcombe, N.
Koskinen, A.
Mattes, J.
Lee, N.
Lee, J.
Dent, L.
Mattaei, K.
Foster, P.
Citation: Journal of Leukocyte Biology, 2004; 75(6):1001-1009
Publisher: Federation Amer Soc Exp Biol
Issue Date: 2004
ISSN: 0741-5400
Statement of
Kristopher Clark, Ljubov Simson, Nicole Newcombe, Aulikki M. L. Koskinen, Joerg Mattes, Nancy A. Lee, James J. Lee, Lindsay A. Dent, Klaus I. Matthaei and Paul S. Foster
Abstract: Eosinophil degranulation is thought to play a pivotal role in the pathogenesis of allergic disorders. Although mouse models of allergic disorders have been used extensively to identify the contribution of eosinophils to disease, ultrastructural evidence of active granule disassembly has not been reported. In this investigation, we characterized the degree of eosinophil activation in the bone marrow, blood, lung tissue, and airways lumen [bronchoalveolar lavage fluid (BALF)] of ovalbumin-sensitized and aero-challenged wild-type and interleukin-5 transgenic mice. Degranulation was most prominent in and primarily compartmentalized to the airways lumen. Eosinophils released granule proteins by the process of piecemeal degranulation (PMD). Accordingly, recruitment and activation of eosinophils in the lung correlated with the detection of cell-free eosinophil peroxidase in BALF and with the induction of airways hyper-reactivity. As in previous studies with human eosinophils, degranulation of isolated mouse cells did not occur until after adherence to extracellular matrix. However, higher concentrations of exogenous stimuli appear to be required to trigger adherence and degranulation (piecemeal) of mouse eosinophils when compared with values reported for studies of human eosinophils. Thus, mouse eosinophils undergo PMD during allergic inflammation, and in turn, this process may contribute to pathogenesis. However, the degranulation process in the allergic lung of mice is primarily compartmentalized to the airway lumen. Understanding the mechanism of eosinophil degranulation in the airway lumen may provide important insights into how this process occurs in human respiratory diseases.
Keywords: asthma
cellular activation
Rights: © 2004 by Society for Leukocyte Biology
DOI: 10.1189/jlb.0803391
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Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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