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Type: Journal article
Title: A duck hepatitis B virus strain with a knockout mutation in the putative X ORF shows similar infectivity and in vivo growth characteristics to wild-type virus
Author: Meier, P.
Scougall, C.
Will, H.
Burrell, C.
Jilbert, A.
Citation: Virology, 2003; 317(2):291-298
Publisher: Academic Press Inc
Issue Date: 2003
ISSN: 0042-6822
Abstract: Hepadnaviruses including human hepatitis B virus (HBV) and duck hepatitis B virus (DHBV) express X proteins, HBx and DHBx, respectively. Both HBx and DHBx are transcriptional activators and modulate cellular signaling in in vitro assays. To test whether the DHBx protein plays a role in virus infection, we compared the in vivo infectivity and growth characteristics of a DHBV3 strain with a stop codon in the X-like ORF (DHBV3-X-K.O.) to those of the wild-type DHBV3 strain. Here we report that the two strains showed no significant difference in (i) their ability to induce infection that resulted in stable viraemia measured by serum surface antigen (DHBsAg) and DHBV DNA, and detection of viral proteins and replicative DNA intermediates in the liver; (ii) the rate of spread of infection in liver and extrahepatic sites after low-dose virus inoculation; and (iii) the ability to produce transient or persistent infection under balanced age/dose conditions designed to detect small differences between the strains. Thus, none of the infection parameters assayed were detectably affected by the X-ORF knockout mutation, raising the question whether DHBx expression plays a physiological role during in vivo infection with wild-type DHBV.
Keywords: Hepadnaviridae
Duck hepatitis B
X protein
In vivo
Outcome of infection
Rate of spread
Description: Copyright © 2003 Elsevier Inc
DOI: 10.1016/j.virol.2003.08.025
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Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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