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|Title:||Bacteriophage P4 Vis protein is needed for prophage excision|
|Citation:||Virology, 2004; 322(1):82-92|
|Publisher:||Academic Press Inc|
|Simona Calì, Elena Spoldi, Daniela Piazzolla, Ian B. Dodd, Francesca Forti, Gianni Dehò and Daniela Ghisotti|
|Abstract:||Upon infection of its host Escherichia coli, satellite bacteriophage P4 can integrate its genome into the bacterial chromosome by Int-mediated site-specific recombination between the attP and the attB sites. The opposite event, excision, may either occur spontaneously or be induced by a superinfecting P2 helper phage. In this work, we demonstrate that the product of the P4 vis gene, a regulator of the P4 late promoters P(LL) and P(sid), is needed for prophage excision. This conclusion is supported by the following evidence: (i) P4 mutants carrying either a frameshift mutation or a deletion of the vis gene were unable to excise both spontaneously or upon P2 phage superinfection; (ii) expression of the Vis protein from a plasmid induced P4 prophage excision; (iii) excision depended on a functional integrase (Int) protein, thus suggesting that Vis is involved in the formation of the excision complex, rather than in the excision recombination event per se; (iv) Vis protein bound P4 DNA in the attP region at two distinct boxes (Box I and Box II), located between the int gene and the attP core region, and caused bending of the bound DNA. Furthermore, we mapped by primer extension the 5' end of the int transcript and found that ectopic expression of Vis reduced its signal intensity, suggesting that Vis is also involved in negative regulation of the int promoter.|
|Keywords:||Coliphages; Integrases; DNA-Binding Proteins; Viral Proteins; DNA, Viral; Cloning, Molecular; Virus Activation; Amino Acid Sequence; Base Sequence; Consensus Sequence; Molecular Sequence Data; Attachment Sites, Microbiological; Promoter Regions, Genetic|
|Appears in Collections:||Molecular and Biomedical Science publications|
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