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Type: Journal article
Title: Compartmentalization of Prion Isoforms Within the Reproductive Tract of the Ram
Author: Ecroyd, Heath William
Sarradin, Pierre
Dacheux, Jean-Louis
Gatti, Jean-Luc
Citation: Biology of Reproduction, 2004; 71:993-1001
Publisher: Society for the Study of Reproduction
Issue Date: 2004
ISSN: 0006-3363
School/Discipline: School of Chemistry and Physics : Chemistry
Statement of
Heath Ecroyd, Pierre Sarradin, Jean-Louis Dacheux, and Jean-Luc Gatti
Abstract: Cellular prion protein (PrpC) is a glycoprotein usually associated with membranes via its glycosylphosphatidylinositol (GPI) anchor. The trans-conformational form of this protein (PrpSC) is the suggested agent responsible for transmissible neurodegenerative spongiform encephalopathies. This protein has been shown on sperm and in the reproductive fluids of males. Antibodies directed against the C-terminal sequence near the GPI-anchor site, an N-terminal sequence, and against the whole protein showed that the Prp isoforms were compartmentalized within the reproductive tract of the ram. Immunoblotting with the three antibodies showed that the complete protein and both N- and C-terminally truncated and glycosylated isoforms are present within cauda epididymal fluid and seminal plasma. Moreover, we demonstrate that in these fluids, the PrpC isoforms are both in a soluble state as well as associated with small membranous vesicles (epididymosomes). We also report that only one major glycosylated 25 kDa C-terminally truncated PrpC isoform is associated with sperm from the testis, cauda epididymis, and semen, and this form is also present in the sperm cytoplasmic droplets that are released during maturation. In sperm, this C-terminal truncated form was found to be associated with membrane lipid rafts present in the mature sperm, suggesting a role for it in the terminal stages of sperm maturation.
Keywords: epididymis; gamete biology; male reproductive tract; sperm; testis
Description: © 2004 by the Society for the Study of Reproduction, Inc.
RMID: RQF0000009
DOI: 10.1095/biolreprod.104.029801
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Appears in Collections:Chemistry publications

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