Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/32911
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Type: Journal article
Title: Metaphyseal factors promote calcium incorporation in physeal chondrocyte cultures
Author: Johnstone, E.
Leane, P.
Byers, S.
Hopwood, J.
Foster, B.
Citation: Journal of Orthopaedic Science, 2000; 5(6):593-599
Publisher: Springer-Verlag Tokyo
Issue Date: 2000
ISSN: 0949-2658
1436-2023
Abstract: Our hypothesis is that physiological mineralization within the mammalian growth plate is a consequence of communication between cartilage chondrocytes and cells within metaphyseal bone. To test this hypothesis, chondrocytes were isolated from the proliferative region of the fetal ovine physis and co-cultured with cells or conditioned medium from cells characteristic of those in metaphyseal bone. The mineralization potential of chondrocytes alone and in the presence of other cells or conditioned medium was determined by 45calcium incorporation. Co-culture of chondrocytes with a crude cell isolate from metaphyseal bone resulted in a stimulation of 45calcium incorporation of 93% above that observed in the individual cell populations alone. Conditioned medium from metaphyseal bone cultures also stimulated 45calcium incorporation. This response to conditioned medium was dose-dependent and stable to 90 degrees C. Vascular endothelial cells and conditioned medium from chondrocyte and osteoblast cultures did not stimulate 45calcium incorporation by physeal chondrocytes. Thus, cells found in the metaphyseal bone produce a soluble factor, which promote calcium incorporation by physeal chondrocytes. The source of this factor is not chondrocytic, osteoblastic, or endothelial in origin.
Keywords: Chondrocytes; Animals; Calcium; Cell Culture Techniques; Calcification, Physiologic
RMID: 0001003023
DOI: 10.1007/s007760070011
Appears in Collections:Paediatrics publications

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