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Type: Journal article
Title: Recombination between paralogues at the rp1 rust resistance locus in maize
Author: Sun, Q.
Collins, N.
Ayliffe, M.
Smith, S.
Drake, J.
Pryor, A.
Hulbert, S.
Citation: Genetics: a periodical record of investigations bearing on heredity and variation, 2001; 158(1):423-438
Publisher: Genetics
Issue Date: 2001
ISSN: 0016-6731
Statement of
Qing Sun, Nicholas C. Collins, Michael Ayliffe, Shavannor M. Smith, Jeff Drake, Tony Pryor, and Scot H. Hulbert
Abstract: Rp1 is a complex rust resistance locus of maize. The HRp1-D haplotype is composed of Rp1-D and eight paralogues, seven of which also code for predicted nucleotide binding site-leucine rich repeat (NBS-LRR) proteins similar to the Rp1-D gene. The paralogues are polymorphic (DNA identities 91–97%), especially in the C-terminal LRR domain. The remaining family member encodes a truncated protein that has no LRR domain. Seven of the nine family members, including the truncated gene, are transcribed. Sequence comparisons between paralogues provide evidence for past recombination events between paralogues and diversifying selection, particularly in the C-terminal half of the LRR domain. Variants selected for complete or partial loss of Rp1-D resistance can be explained by unequal crossing over that occurred mostly within coding regions. The Rp1-D gene is altered or lost in all variants, the recombination breakpoints occur throughout the genes, and most recombinant events (9/14 examined) involved the same untranscribed paralogue with the Rp1-D gene. One recombinant with a complete LRR from Rp1-D, but the amino-terminal portion from another homologue, conferred the Rp1-D specificity but with a reduced level of resistance.
Keywords: Zea mays
DNA Primers
Recombination, Genetic
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Genes, Fungal
Molecular Sequence Data
DOI: 10.1093/genetics/158.1.423
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Appears in Collections:Aurora harvest
Australian Centre for Plant Functional Genomics publications

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