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Type: Journal article
Title: How much effort is required to isolate nuclear microsatellites from plants?
Author: Squirrell, J.
Hollingsworth, P.
Woodhead, M.
Russell, J.
Lowe, A.
Gibby, M.
Powell, W.
Citation: Molecular Ecology, 2003; 12(6):1339-1348
Publisher: Blackwell Publishing Ltd
Issue Date: 2003
ISSN: 0962-1083
Statement of
J. Squirrell, P. M. Hollingsworth, M. Woodhead, J. Russell, A. J. Lowe, M. Gibby and W. Powell
Abstract: The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition of loci at each stage in the process, and the average effort required to obtain 10 working microsatellite primer pairs.
Keywords: enriched library; genotyping; microsatellites; molecular markers; SSRs
Description: The definitive version is available at
RMID: RQF0000152
DOI: 10.1046/j.1365-294X.2003.01825.x
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Appears in Collections:Earth and Environmental Sciences publications
Environment Institute Leaders publications

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