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Type: Journal article
Title: Genetic transformation of major wine grape cultivars of vitis vinifera L.
Author: Iocco, P.
Franks, Tricia K.
Thomas, M. R.
Citation: Transgenic Research, 2001; 10 (2):105-112
Publisher: Kluwer Academic
Issue Date: 2001
ISSN: 0962-8819
School/Discipline: School of Agriculture, Food and Wine : Agricultural and Animal Science
Statement of
P. Iocco, T. Franks and M.R. Thomas
Abstract: We have developed an Agrobacterium-mediated transformation system for a number of important grapevine cultivars used in wine production. Transgenic plants were obtained for the seven cultivars: Cabernet Sauvignon, Shiraz, Chardonnay, Riesling, Sauvignon Blanc, Chenin Blanc and Muscat Gordo Blanco. Embryogenic callus was initiated from anther filaments and genotypic differences were observed for initiation and subsequent proliferation with Chardonnay responding most favourably to culture conditions. The transformation system allowed the recovery of germinating transgenic embryos 10–12 weeks after Agrobacterium inoculation and plants within 18 weeks. Examination of the expression patterns of the green fluorescent protein gene under the control of the CAMV35S promoter in leaf tissue of transgenic plants showed that for up to 35% of plants the pattern was not uniform. The successful transformation of a genetically diverse group of wine grape cultivars indicates that the transformation system may have general application to an even wider range of Vitis vinifera cultivars.
Keywords: Agrobacterium tumefaciens ; grapevine ; somatic embryogenesis ; transgene expression ; transgenic plants ; Vitis vinifera
RMID: RQF0000151
DOI: 10.1023/A:1008989610340
Appears in Collections:Agriculture, Food and Wine publications

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