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dc.contributor.authorYanochko, G.en
dc.contributor.authorYool, A.en
dc.identifier.citationJournal of Neuroscience, 2002; 22(7):2530-2540en
dc.descriptionCopyright © 2002 Society for Neuroscienceen
dc.description.abstractBig brain (bib) is a neurogenic gene that when mutated causes defects in cell fate determination during Drosophila neurogenesis through an unknown mechanism. The protein Big Brain (BIB) has sequence identity with the major intrinsic protein family that includes the water- and ion-conducting aquaporin channels. We show here that BIB expressed heterologously in Xenopus oocytes provides a voltage-insensitive, nonselective cation channel function with permeability to K+ > Na+ tetraethylammonium. The conductance, activated in response to endogenous signaling pathways in BIB-expressing oocytes, is decreased after treatment with 20 µM insulin and is enhanced with 10 µM lavendustin A, a tyrosine kinase inhibitor. Western blot analysis confirms that BIB is tyrosine-phosphorylated. Both tyrosine phosphorylation and the potentiating effect of lavendustin A are removed by partial deletion of the C terminus (amino acids 317-700). Current activation is not observed in control oocytes or in oocytes expressing a nonfunctional mutant (BIB E71N) that appears to be expressed on the plasma membrane by confocal microscopy and Western blotting. These results indicate that BIB can participate in tyrosine kinase-regulated transmembrane signaling and may suggest a role for membrane depolarization in the neurogenic function of BIB in early development.en
dc.description.statementofresponsibilityGina M. Yanochko and Andrea J. Yoolen
dc.publisherSoc Neuroscienceen
dc.subjectmajor intrinsic protein; Xenopus oocyte; tyrosine kinase; voltage clamp; neurogenic; aquaporinen
dc.titleRegulated cationic channel function in Xenopus oocytes expressing Drosophila big brainen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
dc.identifier.orcidYool, A. [0000-0003-1283-585X]en
Appears in Collections:Molecular and Biomedical Science publications

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