Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/35472
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Type: Journal article
Title: Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection
Author: Tacconi, G.
Baldassarre, V.
Collins, N.
Bulgarelli, D.
Stanca, A.
Vale, G.
Citation: Genome, 2006; 49(8):864-872
Publisher: Natl Research Council Canada
Issue Date: 2006
ISSN: 0831-2796
1480-3321
Statement of
Responsibility: 
G Tacconi, V Baldassarre, N C Collins, D Bulgarelli, A M Stanca, G Valè
Abstract: Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, weconducted a polymorphism search in 3 predicted low-copy sequence regions adjacent to the Mlo gene by examining a sample of 4 Mlo and 3 mlo cultivars. Eight singlenucleotide polymorphisms (SNPs) and 1 insertion–deletion (indel) were detected, and easy to use PCR-based markers were developed for typing the SNPs. The PCR markers were used to characterize a collection of 46 Mlo and 25 mlo barley cultivars, identifying 3 distinct mlo-11 haplotypes, 1 mlo-9 haplotype, and 4 Mlo haplotypes. We summarized the haplotype and marker information obtained here and in a previous study to help breeders identify strategies for mlo marker-assisted selection. The ability of the markers to identify mlo-resistant genotypes in segregating populations was demonstrated using 2 resistance-characterized F2 populations derived by 3-way crosses.
Keywords: barley; powdery mildew resistance; mlo; SNPs; marker-assisted selection
RMID: 0020061916
DOI: 10.1139/G06-044
Appears in Collections:Agriculture, Food and Wine publications

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