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Type: Book chapter
Title: Mitochondrial DNA Inheritance after SCNT
Author: Hiendleder, S.
Citation: Advances in experimental medicine and biology: somatic cell nuclear transfer, 2007 / Sutovsky, P. (ed./s), pp.103-116
Publisher: Springer
Publisher Place: LLC, 233 Spring St, New York, New York 10013, USA
Issue Date: 2007
Series/Report no.: Advances in Experimental Medicine and Biology
ISBN: 0387377530
Abstract: Mitochondrial biogenesis and function is under dual genetic control and requires extensive interaction between biparentally inherited nuclear genes and maternally inherited mitochondrial genes. Standard SCNT procedures deprive an oocytes' mitochondrial DNA (mtDNA) of the corresponding maternal nuclear DNA and require it to interact with an entirely foreign nucleus that is again interacting with foreign somatic mitochondria. As a result, most SCNT embryos, -fetuses, and -offspring carry somatic cell mtDNA in addition to recipient oocyte mtDNA, a condition termed heteroplasmy. It is thus evident that somatic cell mtDNA can escape the selective mechanism that targets and eliminates intraspecific sperm mitochondria in the fertilized oocyte to maintain homoplasmy. However, the factors responsible for the large intra- and interindividual differences in heteroplasmy level remain elusive. Furthermore, heteroplasmy is probably confounded with mtDNA recombination. Considering the essential roles of mitochondria in cellular metabolism, cell signalling, and programmed cell death, future experiments will need to assess the true extent and impact of unorthodox mtDNA transmission on various aspects of SCNT success.
Keywords: Oocytes; Cytoplasm; Mitochondria; Animals; DNA, Mitochondrial; Cloning, Organism; Gene Expression Regulation, Developmental; Energy Metabolism; Genes, Mitochondrial; Nuclear Transfer Techniques
RMID: 0020063199
DOI: 10.1007/978-0-387-37754-4_8
Appears in Collections:Agriculture, Food and Wine publications

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