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Type: Conference paper
Title: Magnesium restores altered aquaporin-4 immunoreactivity following traumatic brain injury to a pre-injury state
Author: Ghabriel, M.
Thomas, A.
Vink, R.
Citation: Acta Neurochirurgica: Supplementum, 2006 / Hoff, J., Keep, R., Xi, G., Hua, Y. (ed./s), vol.96, iss.supp, pp.402-406
Publisher: Springer-Verlag Wien
Publisher Place: Sachsenplatz 4-6 PO Box 89 Vienna Austria A-1201
Issue Date: 2006
Series/Report no.: Acta Neurochirurgica Supplements ; Brain Edema XIII
ISBN: 3-211-30712-5
ISSN: 0065-1419
Conference Name: The international symposium on brain edema and tissue injury (1 Jun 2005 - 3 Jun 2005 : Ann Arbor, Michigan)
Editor: Hoff, J.
Keep, R.
Xi, G.
Hua, Y.
Statement of
Mounir N. Ghabriel, A. Thomas and R. Vink
Abstract: Magnesium reduces edema following traumatic brain injury (TBI), although the associated mechanisms are unknown. Recent studies suggest that edema formation after TBI may be related to alterations in aquaporin-4 (AQP4) channels. In this study, we characterize the effects of magnesium administration on AQP4 immunoreactivity following TBI. Male Sprague-Dawley rats were injured by impact-acceleration diffuse TBI and a subgroup was administered 30 mg/kg magnesium sulphate 30 minutes after injury. Animals were fixed by perfusion 5 hours later, which corresponded to the time of maximum edema formation according to previous studies. One half of the brain was cut using a Vibratome and the other half blocked in paraffin wax. Wax and Vibratome sections were immunostained for detection of AQP4 by light and electron microscopy, respectively. In untreated animals, AQP4 immunoreactivity was increased in the subependymal inner glia limitans and the subpial outer glia limitans, and decreased in perivascular astrocytic processes in the cerebrum and brain stem. In contrast, animals treated with magnesium sulphate had AQP4 profiles similar to normal and sham control animals. We conclude that magnesium decreases brain edema formation after TBI, possibly by restoring the polarized state of astrocytes and by down-regulation of AQP4 channels in astrocytes.
Keywords: Cerebral edema
Description: The original publication can be found at
DOI: 10.1007/3-211-30714-1_83
Appears in Collections:Anatomical Sciences publications
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