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Type: Journal article
Title: Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland
Author: Ingman, W.
Wyckoff, J.
Gouon-Evans, V.
Condeelis, J.
Pollard, J.
Citation: Developmental Dynamics, 2006; 235(12):3222-3229
Publisher: Wiley-Liss
Issue Date: 2006
ISSN: 1058-8388
Statement of
Wendy V. Ingman, Jeff Wyckoff, Valerie Gouon-Evans, John Condeelis, Jeffrey W. Pollard
Abstract: Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1op/Csf1op). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1op/Csf1op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds.
Keywords: mammary gland; macrophage; multi-photon microscope; collagen; development
Description: © 2006 Wiley-Liss, Inc
RMID: 0020062075
DOI: 10.1002/dvdy.20972
Published version:
Appears in Collections:Obstetrics and Gynaecology publications

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