Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/35719
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dc.contributor.authorIngman, W.en
dc.contributor.authorWyckoff, J.en
dc.contributor.authorGouon-Evans, V.en
dc.contributor.authorCondeelis, J.en
dc.contributor.authorPollard, J.en
dc.date.issued2006en
dc.identifier.citationDevelopmental Dynamics, 2006; 235(12):3222-3229en
dc.identifier.issn1058-8388en
dc.identifier.issn1097-0177en
dc.identifier.urihttp://hdl.handle.net/2440/35719-
dc.description© 2006 Wiley-Liss, Incen
dc.description.abstractDevelopment of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1op/Csf1op). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1op/Csf1op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds.en
dc.description.statementofresponsibilityWendy V. Ingman, Jeff Wyckoff, Valerie Gouon-Evans, John Condeelis, Jeffrey W. Pollarden
dc.language.isoenen
dc.publisherWiley-Lissen
dc.source.urihttp://www3.interscience.wiley.com/journal/113390093/abstracten
dc.subjectmammary gland; macrophage; multi-photon microscope; collagen; developmenten
dc.titleMacrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary glanden
dc.typeJournal articleen
dc.identifier.rmid0020062075en
dc.identifier.doi10.1002/dvdy.20972en
dc.identifier.pubid51847-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidIngman, W. [0000-0003-3116-2902]en
Appears in Collections:Obstetrics and Gynaecology publications

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