Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/35725
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dc.contributor.authorBrown, H.en
dc.contributor.authorDunning, K.en
dc.contributor.authorRobker, R.en
dc.contributor.authorPritchard, M.en
dc.contributor.authorRussell, D.en
dc.date.issued2006en
dc.identifier.citationDevelopmental Biology, 2006; 300(2):699-709en
dc.identifier.issn0012-1606en
dc.identifier.issn1095-564Xen
dc.identifier.urihttp://hdl.handle.net/2440/35725-
dc.description.abstractMurine ovarian folliculogenesis commences after birth involving oocyte growth, somatic cell differentiation and structural remodeling of follicle stromal boundaries. The extracellular metalloproteinase ADAMTS-1 has activity against proteoglycans and collagen and is produced by the granulosa cells of ovarian follicles. Mice with ADAMTS-1 gene disruption are subfertile due to an unknown mechanism resulting in severely reduced ovulation. Here we show that ADAMTS-1 is necessary for structural remodeling during ovarian follicle growth. A significant reduction in the number of healthy growing follicles and corresponding follicle dysmorphogenesis commencing at the stage of antrum formation was identified in ADAMTS-1-/- ovaries. Morphological analysis and immunostaining of basement membrane components identified stages of follicle dysgenesis from focal disruption in ECM integrity to complete loss of follicular structures. Cells expressing the thecal marker Cyp-17 were lost from dysgenic regions, while oocytes and dispersed cells expressing the granulosa cell marker anti-mullerian hormone persisted in ovarian stroma. Furthermore, we found that the ovarian lymphatic system develops coincidentally with follicular development in early postnatal life but is severely delayed in ADAMTS-1-/- ovaries. These novel roles for ADAMTS-1 in structural maintenance of follicular basement membranes and lymphangiogenesis provide new mechanistic understanding of folliculogenesis, fertility and disease.en
dc.description.statementofresponsibilityHannah M. Brown, Kylie R. Dunning, Rebecca L. Robker, Melanie Pritchard and Darryl L. Russellen
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622816/description#descriptionen
dc.language.isoenen
dc.publisherAcademic Press Inc Elsevier Scienceen
dc.subjectFolliculogenesis; Extracellular matrix remodeling; Infertility; Lymphangiogenesis; Proteaseen
dc.titleRequirement for ADAMTS-1 in extracellular matrix remodeling during ovarian folliculogenesis and lymphangiogenesisen
dc.typeJournal articleen
dc.identifier.rmid0020062262en
dc.identifier.doi10.1016/j.ydbio.2006.10.012en
dc.identifier.pubid51733-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidBrown, H. [0000-0001-6342-3316]en
dc.identifier.orcidDunning, K. [0000-0002-0462-6479]en
dc.identifier.orcidRobker, R. [0000-0002-1538-4604]en
dc.identifier.orcidRussell, D. [0000-0002-4930-7658]en
Appears in Collections:Obstetrics and Gynaecology publications

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