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Type: Journal article
Title: Upregulation of brain-derived neurotrophic factor in the sensory pathway by selective motor nerve injury in adult rats
Author: Li, L.
Xian, C.
Zhong, J.
Zhou, X.
Citation: Neurotoxicity Research, 2006; 9(4):269-283
Publisher: F P Graham Publishing Co.
Issue Date: 2006
ISSN: 1029-8428
Statement of
Li Li, Cory J. Xian, Jin-Hua Zhong, Xin-Fu Zhou
Abstract: Selective motor nerve injury by lumbar 5 ventral root transection (L5 VRT) induces neuropathic pain, but the underlying mechanisms remain unknown. Previously, increased expression and secretion of brain-derived neurotrophic factor (BDNF) had been implicated in injury-induced neuropathic pain in the sensory system. In this study, as a step to examine potential roles of BDNF in L5 VRT-induced neuropathic pain, we investigated BDNF gene and protein expression in adult rats with L5 VRT. L5 VRT induced a dramatic upregulation of BDNF mRNA in intact sensory neurons in the ipsilateral L5 dorsal root ganglia (DRG), in non-neuronal cells in the ipsilateral sciatic nerve, and in motoneurons in the ipsilateral spinal cord. L5 VRT also induced de novo synthesis of BDNF mRNA in spinal dorsal horn neurons and in glial cells in the white matter of the ipsilateral spinal cord. Consistent with the mRNA expression pattern, BDNF protein was also mainly upregulated in all populations of sensory neurons in the ipsilateral L5 DRG and in spinal neurons and glia. Quantitative analysis by ELISA showed that the BDNF content in the DRG and sciatic nerve peaked on day 1 and remained elevated 14 days after L5 VRT. These results suggest that increased BDNF expression in intact primary sensory neurons and spinal cord may be an important factor in the induction of neuropathic pain without axotomy of sensory neurons.
Keywords: Lumbar Vertebrae; Neurons, Afferent; Motor Neurons; Spinal Nerve Roots; Animals; Rats; Rats, Sprague-Dawley; Brain-Derived Neurotrophic Factor; Up-Regulation; Male
RMID: 0020062669
DOI: 10.1007/BF03033317
Appears in Collections:Paediatrics publications

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