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|Title:||Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells|
|Citation:||American Journal of Rhinology & Allergy, 2006; 20(6):652-657|
|Publisher:||Ocean Side Publications Inc|
|Adams, Damian H.; McIntosh, David; Wormald, Peter-John; Cowin, Allison J.|
|Abstract:||Background: Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro. Methods: Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor. Results: All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p ≤ 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells. Conclusion: Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa.|
|Keywords:||Respiratory Mucosa; Cell Line; Epithelial Cells; Humans; Wounds and Injuries; Insulin-Like Growth Factor I; Insulin-Like Growth Factor II; Wound Healing; Cell Movement|
|Appears in Collections:||Surgery publications|
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