Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/36301
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Type: Journal article
Title: Effectiveness of selective genotyping for detection of quantitative trait loci: an analysis of grain and malt quality traits in three barley populations
Author: Ayoub, M.
Mather, D.
Citation: Genome, 2002; 45(6):1116-1124
Publisher: Natl Research Council Canada
Issue Date: 2002
ISSN: 0831-2796
1480-3321
Statement of
Responsibility: 
M. Ayoub and D.E. Mather
Abstract: Marker genotype data and grain and malt quality phenotype data from three barley (Hordeum vulgare L.) mapping populations were used to investigate the feasibility of selective genotyping for detection of quantitative trait loci (QTLs). With selective genotyping, only individuals with high and low phenotypic values for the trait of interest are genotyped. Here, genotyping of 10 to 70% of each population (i.e., 5 to 35% in each tail of the phenotypic distribution) was considered. Genomic positions detected by selective genotyping were compared to QTL position estimates from interval mapping analysis using marker genotype data from the entire population. Selective genotyping reliably detected almost all of the mapped QTLs, often with only 10% of the population genotyped. Selective genotyping also detected spurious QTLs in regions of the genome where no significant QTL had been mapped. Even with additional genotyping to verify putative QTLs, the total genotyping effort for detection of QTLs for a single trait by selective genotyping was usually less than 30% of that required for conventional interval mapping. Simultaneous investigation of two or more traits by selective genotyping would require additional genotyping effort, but could still be worthwhile.
Keywords: Seective genotyping; quantitative trait loci; barley
Description: © 2002 NRC Canada
RMID: 0020063358
DOI: 10.1139/g02-089
Published version: http://rparticle.web-p.cisti.nrc.ca/rparticle/AbstractTemplateServlet?journal=gen&volume=45&year=2002&issue=6&msno=g02-089&calyLang=eng
Appears in Collections:Agriculture, Food and Wine publications

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