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Type: Patent
Title: Ribozyme-mediated synthesis of circular RNA
Author: Randles, John Wesley
Feldstein, Paul A.
Owens, Robert A.
Levy, Laurene
Publisher: The USA as represented by the Secretary of Agriculture
Issue Date: 2001
School/Discipline: School of Agriculture, Food and Wine : Agricultural and Animal Science
Abstract: Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.
Patent #: United States Patent 6210931
Appears in Collections:Agriculture, Food and Wine publications

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