Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/37366
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Type: Journal article
Title: A DNA marker to identify predation of Plutella xylostella (Lep., Plutellidae) by Nabis kinbergii (Hem., Nabidae) and Lycosa sp. (Aranaea, Lycosidae
Author: Ma, J.
Li, D.
Keller, M.
Schmidt, O.
Feng, X.
Citation: Journal of Applied Entomology-Zeitschrift fur Angewandte Entomologie, 2005; 129(6):330-335
Publisher: Blackwell Wissenschafts-Verlag GMBH
Issue Date: 2005
ISSN: 0931-2048
1439-0418
Statement of
Responsibility: 
J. Ma, D. Li, M. Keller, O. Schmidt and X. Feng
Abstract: Predators are important biotic factors in the population dynamics of the diamondback moth, Plutella xylostella. A specific DNA marker was developed to detect P. xylostella in the gut contents of two polyphagous predators, Nabis kinbergii and Lycosa sp. A distinct 275-bp product was amplified by polymerase chain reaction (PCR) from the internal transcribed spacer (ITS-1) of the ribosomal gene of P. xylostella, but not from 11 other arthropod species collected from Brassica fields in South Australia. Fortuitously, the primer set could also amplify DNA products from two species and three varieties of Brassica plants, with the fragment size about 600 bp. When N. kinbergii was analysed after feeding a single fourth instar P. xylostella, 67% of individuals were positive with the 275-bp PCR product up to 16 h after feeding. Likewise, the PCR product was detected in 80% individuals of Lycosa sp. up to 72 h after feeding on a single fourth instar P. xylostella larva. Initial tests of samples collected from the field showed that the predation incidences for both N. kinbergii and Lycosa sp. determined by the 275-bp fragment corresponded to the density of P. xylostella in the field.
Keywords: Lycosa; Nabis kinbergii; Plutella xylostella; gut content analysis; predation
RMID: 0020065035
DOI: 10.1111/j.1439-0418.2005.00977.x
Appears in Collections:Agriculture, Food and Wine publications

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