Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/38770
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis
Author: Shen, Z.
Crotti, T.
McHugh, K.
Matsuzaki, K.
Gravallese, E.
Bierbaum, B.
Goldring, S.
Citation: Arthritis Research and Therapy, 2006; 8(3):1-10
Publisher: BioMed Central Ltd.
Issue Date: 2006
ISSN: 1478-6354
1478-6362
Statement of
Responsibility: 
Zhenxin Shen, Tania N Crotti, Kevin P McHugh, Kenichiro Matsuzaki, Ellen M Gravallese, Benjamin E Bierbaum and Steven R Goldring
Abstract: Prosthetic wear debris-induced peri-implant osteolysis is a major cause of aseptic loosening after total joint replacement. In this condition, wear particles released from the implant components induce a granulomatous inflammatory reaction at the interface between implant and adjacent bone, leading to progressive bone resorption and loss of fixation. The present study was undertaken to characterize definitively the phenotype of osteoclast-like cells associated with regions of peri-implant focal bone resorption and to compare the phenotypic features of these cells with those of mononucleated and multinucleated cells associated with polyethylene wear particles. Peri-implant tissues were obtained from patients undergoing hip revision surgery for aseptic loosening after total joint replacement. Cells were examined for the expression of several markers associated with the osteoclast phenotype using immunohistochemistry, histochemistry, and/or in situ hybridization. CD68 protein, a marker expressed by multiple macrophage lineage cell types, was detected in mononucleated and multinucleated cells associated with polyethylene particles and the bone surface. Cathepsin K and tartrate-resistant acid phosphatase were expressed highly in both mononucleated and multinucleated cells associated with the bone surface. Levels of expression were much lower in cells associated with polyethylene particles. High levels of β3 integrin protein were detected in cells in contact with bone. Multinucleated cells associated with polyethylene particles exhibited faint positive staining. Calcitonin receptor mRNA expression was detected solely in multinucleated cells present in resorption lacunae on the bone surface and was absent in cells associated with polyethylene particles. Our findings provide further evidence that cells expressing the full repertoire of osteoclast phenotypic markers are involved in the pathogenesis of peri-implant osteolysis after total joint replacement. They also demonstrate that foreign body giant cells, although believed to be phenotypically and functionally distinct from osteoclasts, express many osteoclast-associated genes and gene products. However, the levels and patterns of expression of these genes in the two cell types differ. We speculate that, in addition to the role of cytokines and growth factors, the substrate with which these cells interact plays a critical role in their differential phenotypic and functional properties. [Authors' abstract]
Keywords: Macrophages
Osteoclasts
Humans
Bone Resorption
Osteolysis
Acid Phosphatase
Cathepsins
Isoenzymes
Receptors, Calcitonin
RNA, Messenger
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Arthroplasty, Replacement
Prosthesis Implantation
Cathepsin K
Tartrate-Resistant Acid Phosphatase
Rights: © 2006 Shen et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1186/ar1938
Appears in Collections:Aurora harvest 6
Pathology publications

Files in This Item:
File Description SizeFormat 
hdl_38770.pdfPublished version1.48 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.