Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/38986
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Type: Journal article
Title: A novel late embryogenesis abundant protein and peroxidase associated with black point in barley grains
Author: March, T.
Able, J.
Schultz, C.
Able, A.
Citation: Proteomics, 2007; 7(20):3800-3808
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Issue Date: 2007
ISSN: 1615-9853
1615-9861
Statement of
Responsibility: 
Timothy J. March, Jason A. Able, Carolyn J. Schultz and Amanda J. Able
Abstract: Black point of barley grain is a disorder characterised by a brown-black discolouration at the embryo end of the grain. Black point is undesirable to the malting industry and results in significant economic loss annually. To identify proteins associated with barley black point we utilised a proteomic approach with 2-DE to compare proteins from whole grain samples of black pointed and healthy grain. From this comparison two condition-specific proteins were identified: a novel 75 kDa late embryogenesis abundant (LEA) protein and a barley grain peroxidase 1 (BP1) that were specifically more abundant in healthy grain and black pointed grain, respectively. Although LEA protein was less abundant in black pointed grain, LEA gene expression was greater suggesting protein degradation had possibly occurred in black pointed grain. Similarly, the increase in BP1 in black pointed grain could not be explained by gene expression. Western blot analysis also revealed that the identified LEA protein is biotinylated in vivo. The role that each of these proteins might have in black point development is discussed.
Keywords: Barley black point; Grain development; Hordeum vulgare
Description: Published in Proteomics 2007 7:3800-3808 at www.interscience.wiley.com
RMID: 0020073415
DOI: 10.1002/pmic.200700456
Appears in Collections:Agriculture, Food and Wine publications

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