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Type: Conference paper
Title: An investigation of a rapid DNA extraction method for routine MAS in the S.A. Barley Improvement Program
Author: Warner, P.
Karakousis, A.
Schiemann, A.
Eglinton, J.
Langridge, P.
Barr, A.
Citation: Proceedings of the 10th Australian Barley Technical Symposium, 16-20, September 2001: pp.www1-www5
Issue Date: 2002
Conference Name: Australian Barley Technical Symposium (10th : 2001 : Canberra, A.C.T.)
Abstract: A crucial, but limiting step in any MAS program is the reliable and efficient isolation of DNA . While there are numerous DNA extraction protocols and commercial DNA isolation kits available, their cost in barley breeding programs is not economically feasible. Breeders need high-through-put DNA isolation which can be performed on thousands of individuals. An alkaline method was tested for its suitability and it was found that the DNA samples are suitable for only PCR based procedures. Another limitation is that the extracted DNA needs to be processed immediately, and is not suitable for long-term storage.
Appears in Collections:Agriculture, Food and Wine publications
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