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Type: Conference paper
Title: Finding proteins involved with black point of barley using proteomics
Author: March, T.
Schultz, C.
Able, J.
Able, A.
Citation: 12th Australian Barley Technical Symposium Conference Proceedings, 11-14 September 2005: 4p. [CD-ROM]
Publisher: Australian Barley Association
Issue Date: 2005
ISBN: 0975813102
Conference Name: Australian Barley Technical Symposium (12th : 2005 : Hobart, Tasmania)
Abstract: Black point of barley refers to the discolouration of the embryo end of the grain. Downgrading of malting barley to feed grade due to black point results in significant economic loss to the Australian barley industry. Discolouration appears to be a result of enzymatic oxidation of phenolic compounds within the grain with several biochemical pathways involved. To find individual proteins involved in black point formation we are using a broad-based proteomics approach. Using 2D gel electrophoresis, expression profiles of water-soluble and cell wall proteins from black pointed and healthy husk tissue have been compared. Identification of unique proteins involved with black point formation or susceptibility will aid in developing new germplasm with increased tolerance to black point.
Keywords: Proteomics
water-soluble proteins
cell wall proteins
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Appears in Collections:Agriculture, Food and Wine publications
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