Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/39684
Type: Conference paper
Title: Identification of Marker Trait Associations in a Barley Four Way Cross
Author: McMichael, G.
Eglinton, J.
Barr, A.
Chalmers, K.
Citation: 12th Australian Barley Technical Symposium Conference Proceedings, 11-14 September 2005: 4p. [CD-ROM]
Publisher: Australian Barley Association
Issue Date: 2005
ISBN: 0975813102
Conference Name: Australian Barley Technical Symposium (12th : 2005 : Hobart, Tasmania)
Abstract: Until recently, genetic mapping in cereals has been exclusively applied to populations derived from simple crosses. Genetic studies are now targeting broader population structures to take advantage of association mapping and whole genome analysis techniques. This paper presents a study of a Doubled Haploid (DH) population derived from the complex cross, Chieftan/Barque//Manley/VB9104, which is a significant departure from conventional mapping population structures. The population has been extensively phenotyped for malt quality and adaptation characteristics, through evaluation as a mainstream breeding population within the SA Barley Improvement Program (SABIP). Of the 841 (DH) lines, 837 lines were evaluated in double row trials, with 350 individuals promoted to stage one, 70 individuals to stage two and 10 individuals to stage three. One line (WI3408) has subsequently progressed to pilot scale malting and brewing trials, with potential for commercial release. Using high throughput technology the stage one DH lines (350) were extensively screened with fluorescently labelled simple sequence repeats (SSRs) based on their association with the traits of interest for this population. There was a 60 percent rate of polymorphism between one or more parents. In total, 200 SSRs were assayed and analysed using both Gene Flow and Gene Mapper data bases.
Keywords: Barley; whole genome analysis; simple sequence repeats (SSRs)
RMID: 0020073661
Published version: http://www.cdesign.com.au/proceedings_abts2005/
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.