Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/3971
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Type: Journal article
Title: Effect of Emdogain(R) on human periodontal fibroblasts in an in vitro wound-healing model
Author: Rincon, J.
Haase, H.
Bartold, P.
Citation: Journal of Periodontal Research, 2003; 38(3):290-295
Publisher: Blackwell Munksgaard
Issue Date: 2003
ISSN: 0022-3484
1600-0765
Statement of
Responsibility: 
Rincon JC; Haase HR; Bartold PM
Abstract: OBJECTIVE:The aim of this study was to evaluate the influence of Emdogain (EMD) on cultured gingival fibroblasts, periodontal ligament fibroblasts and dermal fibroblasts, using an in vitro model of wound healing. BACKGROUND:Enamel matrix derivative has been demonstrated to promote periodontal regeneration. However, the precise mechanisms by which this agent acts are still unclear. METHODS:The effect of EMD on proliferation of the cells was studied using subconfluent cultures of gingival fibroblasts and periodontal ligament fibroblasts. The cells were made quiescent overnight and then stimulated with various concentrations of EMD (10, 50, 100 and 150 microg/ml) for 24 h. Negative and positive controls were cells cultured in media containing 0.2% and 10% fetal calf serum (FCS). The DNA synthesis was measured by the cellular uptake of [3H]thymidine. For in vitro wounding the cells were cultured, wounded and stimulated with 0.2% FCS, 10% FCS and EMD at a concentration of 20 microg/ml. The percentage of wound fill after treatment was measured after d 1, 4, 6, 12 and 16. The proliferation of cells was also calculated by the extent of incorporation of crystal violet. RESULTS:The results demonstrated that cells in vitro fill an empty space by a combination of proliferation and cell migration. The most rapid closure of a wound area occurred where both proliferation and migration can occur as was seen when wounded cultures were maintained in 10% FCS or at a concentration of 20 microg/ml EMD which promoted proliferation. CONCLUSIONS:Therefore, EMD appears to exert an influence on cells that is compatible with improved wound healing.
Keywords: fibroblasts
periodontal regeneration
enamel matrix
wound healing
cell proliferation
cell migration
Description: Copyright © 2003 Blackwell Munksgaard The definitive version is available at www.blackwell-synergy.com
DOI: 10.1034/j.1600-0765.2003.00610
Appears in Collections:Aurora harvest 2
Dentistry publications

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