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Type: Journal article
Title: Growth hormone regulates osteogenic marker mRNA expression in human periodontal fibroblasts and alveolar bone-derived cells
Author: Haase, H.
Ivanovski, S.
Waters, M.
Bartold, P.
Citation: Journal of Periodontal Research, 2003; 38(4):366-374
Publisher: Blackwell Munksgaard
Issue Date: 2003
ISSN: 0022-3484
Statement of
H. R. Haase, S. Ivanovski, M. J. Waters, P. M. Bartold
Abstract: BACKGROUND:Growth hormone (GH) is a potent regulator of bone formation. The proposed mechanism of GH action is through the stimulation of osteogenic precursor cell proliferation and, following clonal expansion of these cells, promotion of differentiation along the osteogenic lineage. OBJECTIVES:We tested this hypothesis by studying the effects of GH on primary cell populations of human periodontal ligament cells (PLC) and alveolar bone cells (ABC), which contain a spectrum of osteogenic precursors. METHODS:The cell populations were assessed for mineralization potential after long-term culture in media containing beta-glycerophosphate and ascorbic acid, by the demonstration of mineral deposition by Von Kossa staining. The proliferative response of the cells to GH was determined over a 48-h period using a crystal violet dye-binding assay. The profile of the cells in terms of osteogenic marker expression was established using quantitative reverse transcriptase polymerase chain reaction (RT-PCR) for alkaline phosphatase (ALP), osteopontin, osteocalcin, bone sialoprotein (BSP), as well as the bone morphogenetic proteins BMP-2, BMP-4 and BMP-7. RESULTS:As expected, a variety of responses were observed ranging from no mineralization in the PLC populations to dense mineralized deposition observed in one GH-treated ABC population. Over a 48-h period GH was found to be non-mitogenic for all cell populations. Quantitative reverse transcriptase polymerase chain reaction (RT-PCR) BSP mRNA expression correlated well with mineralizing potential of the cells. The change in the mRNA expression of the osteogenic markers was determined following GH treatment of the cells over a 48-h period. GH caused an increase in ALP in most cell populations, and also in BMP expression in some cell populations. However a decrease in BSP, osteocalcin and osteopontin expression in the more highly differentiated cell populations was observed in response to GH. CONCLUSION:The response of the cells indicates that while long-term treatment with GH may promote mineralization, short-term treatment does not promote proliferation of osteoblast precursors nor induce expression of late osteogenic markers.
Keywords: Alveolar Process
Periodontal Ligament
Human Growth Hormone
Alkaline Phosphatase
Transforming Growth Factor beta
Bone Morphogenetic Proteins
RNA, Messenger
Cell Culture Techniques
Cell Division
Cell Differentiation
Gene Expression Regulation
Calcification, Physiologic
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 7
Integrin-Binding Sialoprotein
Description: Copyright © 2003 Blackwell Munksgaard The definitive version is available at
DOI: 10.1034/j.1600-0765.2003.00655
Published version:
Appears in Collections:Aurora harvest 2
Dentistry publications

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