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Type: Journal article
Title: Tissue engineering for bone regeneration using differentiated alveolar bone cells in collagen scaffolds
Author: Xiao, Y.
Qian, H.
Young, W.
Bartold, P.
Citation: Tissue Engineering, 2003; 9(6):1167-1177
Publisher: Mary Ann Liebert Inc Publ
Issue Date: 2003
ISSN: 1076-3279
Statement of
Y. Xiao, H. Qian, W.G. Young and P.M. Bartold
Abstract: Regeneration of osseous defects by a tissue-engineering approach provides a novel means of treatment utilizing cell biology, materials science, and molecular biology. In this study the concept of tissue engineering was tested with collagen type I matrices seeded with cells with osteogenic potential and implanted into sites where osseous damage had occurred. Explant cultures of cells from human alveolar bone and gingiva were established. When seeded into a three-dimensional type I collagen-based scaffold, the bone-derived cells maintained their osteoblastic phenotype as monitored by mRNA and protein levels of the bone-related proteins including bone sialoprotein, osteocalcin, osteopontin, bone morphogenetic proteins 2 and 4, and alkaline phosphatase. These in vitro-developed matrices were implanted into critical-size bone defects in skulls of immunodeficient (SCID) mice. Wound healing was monitored for up to 4 weeks. When measured by microdensitometry the bone density within defects filled with osteoblast-derived matrix was significantly higher compared with defects filled with either collagen scaffold alone or collagen scaffold impregnated with gingival fibroblasts. New bone formation was found at all the sites treated with the osteoblast-derived matrix at 28 days, whereas no obvious new bone formation was identified at the same time point in the control groups. In situ hybridization for the human-specific Alu gene sequence indicated that the newly formed bone tissue resulted from both transplanted human osteoblasts and endogenous mesenchymal stem cells. The results indicate that cells derived from human alveolar bone can be incorporated into bioengineered scaffolds and synthesize a matrix, which on implantation can induce new bone formation.
Keywords: Alveolar Process
Cells, Cultured
Skull Fractures
Membranes, Artificial
Cell Culture Techniques
Tissue Engineering
Bone Regeneration
Wound Healing
Cell Differentiation
Bone Density
DOI: 10.1089/10763270360728071
Appears in Collections:Aurora harvest 6
Dentistry publications

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