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|Title:||Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells|
|Citation:||Journal of Cellular Physiology, 2001; 186(3):448-456|
|W. Worapamorn, H.R. Haase, H. Li, and P.M. Bartold|
|Abstract:||Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-beta1, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TGF-beta1 and IL-1beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell-surface proteoglycans.|
|Keywords:||Cells, Cultured; Cell Membrane; Periodontal Ligament; Humans; Proteoglycans; Growth Substances; Fibroblast Growth Factor 2; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-sis; Transforming Growth Factor beta; beta 2-Microglobulin; Membrane Glycoproteins; Receptors, Transforming Growth Factor beta; Recombinant Proteins; Interleukin-1; Cytokines; Reverse Transcriptase Polymerase Chain Reaction; Cell Division; Cell Movement; Transcription, Genetic; Gene Expression Regulation; Heparan Sulfate Proteoglycans; Syndecan-1; Syndecans; Syndecan-4; Syndecan-2; Interferon-gamma; Becaplermin|
|Description:||Published online in Wiley InterScience, 23 January 2001|
|Rights:||© 2001 Wiley-Liss, Inc.|
|Appears in Collections:||Dentistry publications|
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