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|Title:||Leukocyte Migration in Adipose Tissue of Mice Null for ICAM-1 and Mac-1 Adhesion Receptors|
|Citation:||Obesity, 2004; 12(6):936-940|
|Publisher:||North Amer Assoc Study Obesity|
|Rebecca L. Robker, Robert G. Collins, Arthur L. Beaudet, Harry J. Mersmann and C. Wayne Smith|
|Abstract:||Objective: To determine whether the leukocyte adhesion receptors ICAM-1 and Mac-1, regulators of immune cell migration, have an intrinsic role within adipose tissue by 1) analyzing the expression of ICAM-1 in adipose tissue, 2) identifying leukocyte populations within adipose tissue, and 3) determining whether ICAM-1 and Mac-1 mutant mice exhibit abnormal numbers of adipose tissue leukocytes. Research Methods and Procedures: Wild-type, ICAM-1-/-, and Mac-1-/- mice were fed a long-term high-fat diet. ICAM-1 expression was analyzed by Northern blot and immunohistochemistry. Leukocytes within adipose tissue were identified by immunohistochemistry and flow cytometry. Results: ICAM-1 was expressed in adipose tissue and localized to the vascular endothelium. Macrophages and lymphocytes were prevalent within the stromal-vascular cell fraction of adipose tissue, and gender-specific differences were observed, with adipose tissue from female mice containing significantly more macrophages than tissue from male mice. Numbers of leukocytes in ICAM-1-/- and Mac-1-/- mice were not different from wild-types, however, indicating that these adhesion receptors are not required for leukocyte migration into adipose tissue. Discussion: Our results documented leukocyte populations within adipose tissue, which may be involved in the development of heightened inflammation that is characteristic of obesity.|
|Keywords:||Endothelium, Vascular; Adipose Tissue; Leukocytes; Animals; Mice, Knockout; Mice; Intercellular Adhesion Molecule-1; Macrophage-1 Antigen; RNA; Blotting, Northern; Flow Cytometry; Immunohistochemistry; Cell Movement; Gene Expression Regulation; Female; Male|
|Rights:||© 2004 The Obesity Society|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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