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https://hdl.handle.net/2440/42308
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dc.contributor.author | Robker, R. | - |
dc.contributor.author | Collins, R. | - |
dc.contributor.author | Beaudet, A. | - |
dc.contributor.author | Mersmann, H. | - |
dc.contributor.author | Smith, C. | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Obesity, 2004; 12(6):936-940 | - |
dc.identifier.issn | 1930-7381 | - |
dc.identifier.issn | 1550-8528 | - |
dc.identifier.uri | http://hdl.handle.net/2440/42308 | - |
dc.description.abstract | Objective: To determine whether the leukocyte adhesion receptors ICAM-1 and Mac-1, regulators of immune cell migration, have an intrinsic role within adipose tissue by 1) analyzing the expression of ICAM-1 in adipose tissue, 2) identifying leukocyte populations within adipose tissue, and 3) determining whether ICAM-1 and Mac-1 mutant mice exhibit abnormal numbers of adipose tissue leukocytes. Research Methods and Procedures: Wild-type, ICAM-1-/-, and Mac-1-/- mice were fed a long-term high-fat diet. ICAM-1 expression was analyzed by Northern blot and immunohistochemistry. Leukocytes within adipose tissue were identified by immunohistochemistry and flow cytometry. Results: ICAM-1 was expressed in adipose tissue and localized to the vascular endothelium. Macrophages and lymphocytes were prevalent within the stromal-vascular cell fraction of adipose tissue, and gender-specific differences were observed, with adipose tissue from female mice containing significantly more macrophages than tissue from male mice. Numbers of leukocytes in ICAM-1-/- and Mac-1-/- mice were not different from wild-types, however, indicating that these adhesion receptors are not required for leukocyte migration into adipose tissue. Discussion: Our results documented leukocyte populations within adipose tissue, which may be involved in the development of heightened inflammation that is characteristic of obesity. | - |
dc.description.statementofresponsibility | Rebecca L. Robker, Robert G. Collins, Arthur L. Beaudet, Harry J. Mersmann and C. Wayne Smith | - |
dc.language.iso | en | - |
dc.publisher | North Amer Assoc Study Obesity | - |
dc.rights | © 2004 The Obesity Society | - |
dc.source.uri | http://dx.doi.org/10.1038/oby.2004.114 | - |
dc.subject | Endothelium, Vascular | - |
dc.subject | Adipose Tissue | - |
dc.subject | Leukocytes | - |
dc.subject | Animals | - |
dc.subject | Mice, Knockout | - |
dc.subject | Mice | - |
dc.subject | Intercellular Adhesion Molecule-1 | - |
dc.subject | Macrophage-1 Antigen | - |
dc.subject | RNA | - |
dc.subject | Blotting, Northern | - |
dc.subject | Flow Cytometry | - |
dc.subject | Immunohistochemistry | - |
dc.subject | Cell Movement | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.title | Leukocyte Migration in Adipose Tissue of Mice Null for ICAM-1 and Mac-1 Adhesion Receptors | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1038/oby.2004.114 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robker, R. [0000-0002-1538-4604] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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