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Type: Journal article
Title: Differential expression of oxygen-regulated genes in bovine blastocysts
Author: Harvey, A.
Navarrete Santos, A.
Kirstein, M.
Kind, K.
Fischer, B.
Thompson, J.
Citation: Molecular Reproduction and Development, 2007; 74(3):290-299
Publisher: Wiley-Liss
Issue Date: 2007
ISSN: 1040-452X
Abstract: Low oxygen conditions (2%) during post-compaction culture of bovine blastocysts improve embryo quality, which is associated with a small yet significant increase in the expression of glucose transporter 1 (GLUT-1), suggesting a role of oxygen in embryo development mediated through oxygen-sensitive gene expression. However, bovine embryos to at least the blastocyst stage lack a key regulator of oxygen-sensitive gene expression, hypoxia-inducible factor 1α (HIF1α). A second, less well-characterized protein (HIF2α) is, however, detectable from the 8-cell stage of development. Here we use differential display to determine additional gene targets in bovine embryos in response to low oxygen conditions. While development to the blastocyst stage was unaffected by the oxygen concentration used during post-compaction culture, differential display identified oxygen-regulation of myotrophin and anaphase promoting complex 1 expression, with significantly lower levels observed following culture under 20% oxygen than 2% oxygen. These results further support the hypothesis that the level of gene expression of specific transcripts by bovine embryos alters in response to changes in the oxygen environment post-compaction. Specifically, we have identified two oxygen-sensitive genes that are potentially regulated by HIF2 in the bovine blastocyst.
Keywords: Cells, Cultured; Blastocyst; Animals; Cattle; Oxygen; RNA, Messenger; Embryo Culture Techniques; Polymerase Chain Reaction; Cell Cycle; Gene Expression Regulation, Developmental; Molecular Sequence Data; Female
RMID: 0020070071
DOI: 10.1002/mrd.20617
Appears in Collections:Obstetrics and Gynaecology publications

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