Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/42884
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dc.contributor.authorHarvey, A.en
dc.contributor.authorNavarrete Santos, A.en
dc.contributor.authorKirstein, M.en
dc.contributor.authorKind, K.en
dc.contributor.authorFischer, B.en
dc.contributor.authorThompson, J.en
dc.date.issued2007en
dc.identifier.citationMolecular Reproduction and Development, 2007; 74(3):290-299en
dc.identifier.issn1040-452Xen
dc.identifier.issn1098-2795en
dc.identifier.urihttp://hdl.handle.net/2440/42884-
dc.description.abstractLow oxygen conditions (2%) during post-compaction culture of bovine blastocysts improve embryo quality, which is associated with a small yet significant increase in the expression of glucose transporter 1 (GLUT-1), suggesting a role of oxygen in embryo development mediated through oxygen-sensitive gene expression. However, bovine embryos to at least the blastocyst stage lack a key regulator of oxygen-sensitive gene expression, hypoxia-inducible factor 1α (HIF1α). A second, less well-characterized protein (HIF2α) is, however, detectable from the 8-cell stage of development. Here we use differential display to determine additional gene targets in bovine embryos in response to low oxygen conditions. While development to the blastocyst stage was unaffected by the oxygen concentration used during post-compaction culture, differential display identified oxygen-regulation of myotrophin and anaphase promoting complex 1 expression, with significantly lower levels observed following culture under 20% oxygen than 2% oxygen. These results further support the hypothesis that the level of gene expression of specific transcripts by bovine embryos alters in response to changes in the oxygen environment post-compaction. Specifically, we have identified two oxygen-sensitive genes that are potentially regulated by HIF2 in the bovine blastocyst.en
dc.language.isoenen
dc.publisherWiley-Lissen
dc.subjectCells, Cultured; Blastocyst; Animals; Cattle; Oxygen; RNA, Messenger; Embryo Culture Techniques; Polymerase Chain Reaction; Cell Cycle; Gene Expression Regulation, Developmental; Molecular Sequence Data; Femaleen
dc.titleDifferential expression of oxygen-regulated genes in bovine blastocystsen
dc.typeJournal articleen
dc.identifier.rmid0020070071en
dc.identifier.doi10.1002/mrd.20617en
dc.identifier.pubid49328-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]en
Appears in Collections:Obstetrics and Gynaecology publications

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