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Type: Journal article
Title: Human cathelicidin antimicrobial peptide is upregulated in the eosinophilic mucus subgroup of chronic rhinosinusitis patients
Author: Ooi, E.
Wormald, P.
Carney, A.
James, C.
Tan, L.
Citation: American Journal of Rhinology and Allergy, 2007; 21(4):395-401
Publisher: Ocean Side Publications Inc
Issue Date: 2007
ISSN: 1945-8924
Abstract: Background: Eosinophilic mucus chronic rhinosinusitis (EMCRS) patients are a subgroup of CRS with a poorer prognosis due to frequent recurrences of disease. The cathelicidin antimicrobial peptide (CAMP) is an important innate defense peptide but its role in CRS is not well characterized. The purpose of this study was to investigate CAMP mRNA and protein expression from EMCRS, CRS, and normal control patients. Methods: Biopsy specimens of nasal mucosa and nasal polyps were taken from 59 CRS patients and 9 controls. CAMP mRNA and protein levels were analyzed by real-time quantitative reverse-transcriptase polymerase chain reaction, immunoassay, Western blot, and immunohistochemistry. Results: The expression of CAMP mRNA was significantly increased in EMCRS patients compared with CRS patients (p = 0.0004). By immunohistochemistry, expression of CAMP was localized to nasal epithelial, submucosal glands, and inflammatory subepithelial cells. Western blotting confirmed the presence of CAMP in EMCRS, CRS, and control patients. However, we did not detect statistically significant differences in the protein levels in tissue homogenates between EMCRS, CRS, and control patients. Conclusion: This study shows expression of CAMP in nasal mucosa supporting its role in innate defenses against inhaled pathogens. Although CAMP mRNA was up-regulated in EMCRS patients, there were no statistically significant differences in protein levels in the nasal mucosa of EMCRS compared with CRS patients and controls.
Keywords: Nasal Mucosa
Chronic Disease
Antimicrobial Cationic Peptides
RNA, Messenger
Blotting, Western
Reverse Transcriptase Polymerase Chain Reaction
Aged, 80 and over
Middle Aged
DOI: 10.2500/ajr.2007.21.3048
Appears in Collections:Aurora harvest
Surgery publications

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