Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/42945
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Type: Journal article
Title: EphB/ephrin-B interaction mediates adult stem cell attachment spreading and migration: Implications for dental tissue repair
Author: Arthur, A.
Shi, S.
Sun, T.
Bartold, P.
Koblar, S.
Gronthos, S.
Citation: Stem Cells, 2007; 25(1):156-164
Publisher: Alphamed Press
Issue Date: 2007
ISSN: 1066-5099
1549-4918
Organisation: Centre for the Molecular Genetics of Development
Statement of
Responsibility: 
Agnieszka Stokowski, Songtao Shi, Tao Sun, Peter Mark Bartold, Simon Andrea Koblar, Stan Gronthos
Abstract: Human adult dental pulp stem cells (DPSCs) reside predominantly within the perivascular niche of dental pulp and are thought to originate from migrating neural crest cells during development. The Eph family of receptor tyrosine kinases and their ligands, the ephrin molecules, play an essential role in the migration of neural crest cells during development and stem cell niche maintenance. The present study examined the expression and function of the B-subclass Eph/ephrin molecules on DPSCs. Multiple receptors were primarily identified on DPSCs within the perivascular niche, whereas ephrin-B1 and ephrin-B3 were expressed by the surrounding pulp tissue. EphB/ephrin-B bidirectional signaling inhibited cell attachment and spreading, predominately via the mitogen-activated protein kinase (MAPK) pathway for forward signaling and phosphorylation of Src family tyrosine kinases via reverse ephrin-B signaling. DPSC migration was restricted through unidirectional ephrin-B1-activated EphB forward signaling, primarily signaling through the MAPK pathway. Furthermore, we observed that ephrin-B1 was downregulated in diseased adult teeth compared with paired uninjured controls. Collectively, these studies suggest that EphB/ephrin-B molecules play a role in restricting DPSC attachment and migration to maintain DPSCs within their stem cell niche under steady-state conditions. These results may have implications for dental pulp development and regeneration.
Keywords: Eph
Ephrin
MSC
Dental pulp stem cell
Migration
Adhesion
Spreading
Description: Copyright © 2007 AlphaMed Press
DOI: 10.1634/stemcells.2006-0373
Appears in Collections:Aurora harvest 6
Centre for the Molecular Genetics of Development publications
Dentistry publications

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