Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/43145
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Type: Journal article
Title: Collagen loss and impaired wound healing is associated with c-Myb deficiency
Author: Kopecki, Z.
Luchetti, M.
Adams, D.
Strudwick, X.
Mantamadiotis, T.
Stoppacciaro, A.
Gabrielli, A.
Ramsay, R.
Cowin, A.
Citation: Journal of Pathology, 2007; 211(3):351-361
Publisher: John Wiley & Sons Ltd
Issue Date: 2007
ISSN: 0022-3417
1096-9896
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Responsibility: 
Z Kopecki, MM Luchetti, DH Adams, X Strudwick, T Mantamadiotis, A Stoppacciaro, A Gabrielli, RG Ramsay, AJ Cowin
Abstract: Collagen type I serves as an abundant structural and signalling component of skin. It is also an established target gene of the transcription factor, c-Myb. When c-myb-/- embryos were examined it was observed that their skin was markedly thinner than normal. Importantly, immunohistochemical investigation showed complete absence of collagen type I. Although these homozygous knock-out embryos fail to develop beyond day 15, fibroblasts established from these embryos (mouse embryonic fibroblasts [MEFs]) show defective proliferative responses. Furthermore, in vitro scratch wound assays demonstrated that these c-myb-/- MEFs also exhibit slower closure than their wild-type counterparts. Embryonic lethality has meant that examination of the role of c-Myb in adult mouse skin has not been reported to date. However, in view of the abundance of collagen type I in normal skin, its role in skin integrity and the in vitro data showing proliferative and migration defects in c-myb-/- MEFs, we investigated the consequences of heterozygous c-myb loss in adult mice on the complex process of skin repair in response to injury. Our studies clearly demonstrate that heterozygous c-myb deficiency has a functional effect on wound repair, collagen type I levels and, in response to wounding, transforming growth factor-beta1 (an important collagen stimulating factor) induction expression is aberrantly high. Manipulation of c-Myb may therefore provide new therapeutic opportunities for improving wound repair while uncontrolled expression may underpin some fibrotic disorders.
Keywords: c-myb
collagen
TGF-β1
wound healing
fibrosis
Rights: Copyright © 2007 Pathological Society of Great Britain and Ireland.
DOI: 10.1002/path.2113
Appears in Collections:Aurora harvest
Paediatrics publications

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