Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/43410
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Type: Journal article
Title: Low CD4 T cell immunity to pneumolysin is associated with nasopharyngeal carriage of pneumococci in children
Author: Zhang, Q.
Bagrade, L.
Bernatoniene, J.
Clarke, E.
Paton, J.
Mitchell, T.
Nunez, D.
Finn, A.
Citation: Journal of Infectious Diseases, 2007; 195(8):1194-1202
Publisher: Univ Chicago Press
Issue Date: 2007
ISSN: 0022-1899
1537-6613
Statement of
Responsibility: 
Qibo Zhang, Linda Bagrade, Jolanta Bernatoniene, Ed Clarke, James C. Paton, Tim J. Mitchell, Desmond A. Nunez, and Adam Finn
Abstract: <h4>Background</h4>Recent studies in mice have suggested that T cell immunity may be protective against pneumococcal infection.<h4>Methods</h4>CD4 T cell proliferative responses to the pneumococcal proteins pneumolysin (Ply), Ply toxoid (F433), and choline-binding protein A were investigated in peripheral blood mononuclear cells (PBMCs) and adenoidal mononuclear cells (MNCs) obtained from children undergoing adenoidectomy.<h4>Results</h4>Ply and F433 induce significant proliferation of CD4 T cells in both PBMCs and adenoidal MNCs, and both memory and naive phenotypes of CD4 T cells proliferated after stimulation. In PBMCs, CD4 T cell proliferation induced by Ply and F433, which was associated with increased production of interferon (IFN)- gamma and tumor necrosis factor (TNF)- alpha , was significantly lower in children who were culture positive for pneumococcus than in those who were culture negative for pneumococcus (P<.05). Between groups, no such difference was observed in adenoidal MNC CD4 T cell proliferation, which was associated with production of IFN- gamma and interleukin (IL)-10. The CD4 T cell proliferation induced by Ply and F433 was inhibited by antibodies to Toll-like receptor 4.<h4>Conclusion</h4>These data suggest that Ply induces CD4 T cell proliferative responses with production of IFN- gamma and TNF- alpha in PBMCs or of IFN- gamma and IL-10 in adenoidal MNCs, which may be important in modulating pneumococcal carriage in children.
Keywords: Nasopharynx; CD4-Positive T-Lymphocytes; Cells, Cultured; Humans; Streptococcus pneumoniae; Pneumococcal Infections; Tumor Necrosis Factor-alpha; Bacterial Proteins; Streptolysins; Antibodies, Bacterial; Carrier State; Cell Proliferation; Time Factors; Child; Child, Preschool; Female; Male; Toll-Like Receptors; Interferon-gamma
Description: © 2007 by the Infectious Diseases Society of America. All rights reserved.
RMID: 0020070532
DOI: 10.1086/512617
Published version: http://www.journals.uchicago.edu/doi/abs/10.1086/512617
Appears in Collections:Molecular and Biomedical Science publications

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