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Type: Journal article
Title: A rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos
Author: Tucker, B.
Lardelli, M.
Citation: Zebrafish, 2007; 4(2):113-116
Publisher: Mary Ann Liebert, Inc. Publishers
Issue Date: 2007
ISSN: 1545-8547
Organisation: Centre for the Molecular Genetics of Development
Statement of
Ben Tucker and Michael Lardelli
Abstract: The ability to easily analyze apoptosis is important in studies of molecular cell biology and to evaluate the relative toxicity of different treatments or environments. This is particularly the case when substances such as morpholino oligonucleotides are injected into embryos, as such treatments can cause widespread, complex patterns of apoptosis. Zebrafish embryos are well suited for cell biological and environmental toxicity analyses, but the need remains for a simple method that can analyze levels of apoptosis in a statistically significant number of embryos. Here we present a "group fluorescence" method for rapid, large-scale analysis of relative levels of apoptosis based on densitometric techniques.
Keywords: Embryo, Nonmammalian
Acridine Orange
Oligonucleotides, Antisense
Fluorescent Dyes
Staining and Labeling
Description: Copyright © 2007 Mary Ann Liebert, Inc.
DOI: 10.1089/zeb.2007.0508
Appears in Collections:Aurora harvest 6
Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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