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dc.contributor.authorRobinson, L.-
dc.contributor.authorJuttner, J.-
dc.contributor.authorMilligan, A.-
dc.contributor.authorLahnstein, J.-
dc.contributor.authorEglinton, J.-
dc.contributor.authorEvans, D.-
dc.identifier.citationJournal of Cereal Science, 2007; 45(3):343-352-
dc.descriptionCopyright © 2006 Elsevier Ltd All rights reserved.-
dc.description.abstractPrevious pilot brewing trials have demonstrated that in the absence of a molecular weight (MW) ~12,000 (barley silica eluate (SE) protein), the beer brewed from the malt of these SE −ve varieties formed less haze after accelerated ageing than beers brewed using SE +ve malt varieties. The previously described SE protein was characterised using comparative two-dimensional (2-D) gel electrophoresis immunoblots of barley seed extracts from both SE +ve and SE −ve varieties. The SE protein spot identified was excised and its partial sequence determined, after in-gel cleavage using trypsin and separation of the resulting fragments by reversed-phase high performance liquid chromatography (HPLC). N-terminal sequence analysis of the tryptic peptides from SE +ve and SE −ve varieties identified the SE protein as the barley trypsin inhibitor-CMe precursor (BTI-CMe). The mature BTI-CMe protein is 13.3 kDa and its functional gene is located on chromosome 3H. Cloning of the BTI-CMe protein demonstrated that both SE −ve and SE +ve barley varieties contain a BTI-CMe protein family member that is similar but has a consistently different sequence, primarily in the last 30 amino acid residues of their C-termini.-
dc.description.statementofresponsibilityLouise H. Robinson, Peter Healy, Doug C. Stewart, Jason K. Eglinton, Chris M. Ford and David E. Evans-
dc.publisherAcademic Press Ltd Elsevier Science Ltd-
dc.subjectBarley trypsin inhibitor-
dc.titleThe identification of a barley haze active protein that influences beer haze stability: Cloning and characterisation of the barley SE protein as a barley trypsin inhibitor of the chloroform/methanol type-
dc.typeJournal article-
dc.identifier.orcidLahnstein, J. [0000-0002-4202-1403]-
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest

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