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Type: Journal article
Title: Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets
Author: Hodge, G.
Hodge, S.
Reynolds, P.
Holmes, M.
Citation: Transplant Infectious Disease Online, 2008; 10(2):99-105
Publisher: Wiley-Blackwell Munksgaard
Issue Date: 2008
ISSN: 1399-3062
Statement of
G. Hodge, S. Hodge, P.N. Reynolds and M. Holmes
Abstract: Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with ‘culture-negative’ BAL and 13 patients with ‘culture-positive’ BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-α (TNF-α) in CD8+ T cells (but not CD4+) in ‘culture-positive’ compared with ‘culture-negative’ transplant patients. There was no difference in blood Th1 T-cell cytokines between ‘culture-positive’ compared with ‘culture-negative’ transplant patients. A decrease in Th1 cytokines IL-2 and TNF-α in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients.
Keywords: T-Lymphocyte Subsets; T-Lymphocytes; CD4-Positive T-Lymphocytes; Th1 Cells; CD8-Positive T-Lymphocytes; Bronchoalveolar Lavage Fluid; Humans; Lung Diseases; Tumor Necrosis Factor-alpha; Interleukin-2; Cytokines; Bronchoscopy; Lung Transplantation; Flow Cytometry; Case-Control Studies; Transplantation Immunology; Immunocompromised Host; Adult; Female; Male
Description: The definitive version is available at
Provenance: Published online in 2007.
RMID: 0020073889
DOI: 10.1111/j.1399-3062.2007.00236.x
Appears in Collections:Paediatrics publications

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